Exploring Morphology and Drug Interactions in Pancreatic Cancer with 3D Cell Culture

Loading...
Thumbnail Image
File version
Primary Supervisor

Avery, Vicky

Other Supervisors

Stevenson, Graeme

Editor(s)
Date
2014
Size
File type(s)
Location
License
Abstract

Pancreatic cancer continues to have one of the poorest prognoses amongst all cancers, with a 95% mortality rate. Standard of care chemotherapy has failed to provide significant clinical benefits, which has led to the development of targeted agents against validated signalling pathways. However, to date the approach of targeted agents alone, or in combination with traditional chemotherapeutics, has failed to significantly improve the prognosis for pancreatic cancer patients. The current standard of care chemotherapy for advanced pancreatic cancer provides only a modest increase in survival of several months. Models that improve the predictive potential of drug discovery programs and gain greater insights into the complexity of tumour biology are therefore urgently required. To better understand the mechanisms influencing the anti-cancer activities of current and novel therapies, we have developed a 3D in vitro micro-tumour cell culture model. Current in vitro models utilising cell monolayer cultures are unable to recapitulate the biological and physiological complexities of the in vivo pancreatic tumour microenvironment and may be poor predictors of drug efficacy. Pancreatic adenocarcinomas are characterised as having a highly dense and poorly vascularised stroma that is made up of extracellular matrix (ECM) components and host cells. This complex tumour microenvironment has been implicated in the chemoresistance profiles observed in pancreatic tumours.

Journal Title
Conference Title
Book Title
Edition
Volume
Issue
Thesis Type

Thesis (PhD Doctorate)

Degree Program

Doctor of Philosophy (PhD)

School

Eskitis Institute for Cell and Molecular Therapies

Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement

The author owns the copyright in this thesis, unless stated otherwise.

Item Access Status

Public

Note

In order to comply with copyright Figure 1.12 has not been published here.

Access the data
Related item(s)
Subject

Pancreatic cancer

Chemotherapy

Cell monolayer cultures

Extracellular matrix (ECM) components and host cells

Pancreatic tumours

Persistent link to this record
Citation