Moraxella bovoculi is a Gram-negative microorganism that has shown potential to aide in the pathogenesis of infectious bovine keratoconjunctivitis (IBK) or 'pink-eye' in cattle. An ocular disease, IBK has shown to cause a significant economic loss to the cattle and dairy industry with reported losses of AUD 21 million annually to the beef industry in Australia.1 Infected animals show symptoms of keratitis, conjunctivitis, corneal ulceration and in severe cases, IBK can lead to permanent blindness.2 Currently, the only known cause for IBK is the Gram-negative bacterium Moraxella bovis. However, M. bovoculi is a recently isolated bacterium from calves which could also play a role in the pathogenesis of IBK with reports suggesting it may play a role in host colonisation.2-4 Currently, no investigations on the lipooligosaccharide (LOS) structure of M. bovoculi have been completed which is a major virulence factor found in Gram-negative bacteria.3,5,6 This following study aimed to further understand the biological role of LOS in M. bovoculi as well as compare it to the previously studied Moraxella species, M. bovis and M. catarrhalis. The first aim investigated the growth profile and biological activity of Moraxella bovoculi. The second aim investigated the extraction and purification of the LOS of M. bovoculi leading to the eventual analysis of the oligosaccharide structure using NMR spectroscopy. [...]