Molecular Characterization of DNA Double Strand Breaks with Tip-Enhanced Raman Scattering

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Lipiec, Ewelina
Sekine, Ryo
Bielecki, Jakub
Kwiatek, Wojciech M.
Wood, Bayden R.
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2014
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Abstract

DNA double strand breaks (DSBs) are deadly lesions that can lead to genetic defects and cell apoptosis. Techniques that directly detect DNA DSBs include scanning electron microscopy, atomic force microscopy (AFM), and fluorescence based approaches. While these techniques can be used to identify DSBs they provide no information on the molecular events occurring at the break. Tip‐enhanced Raman scattering (TERS) can provide molecular information from DNA at the nanoscale and in combination with AFM provides a new way to visualize and characterize the molecular structure of DSBs. DSBs result from cleavage at the 3’‐ and 5’‐bonds of deoxyribose upon exposure to UVC radiation based on the observation of POH and methyl/methylene deformation modes enhanced in the TERS spectra. It is hypothesized that strand fragments are hydrogen‐terminated at the lesion, indicating the action of free radicals during photon exposure.

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Angewandte Chemie

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126

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1

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© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Molecular Characterization of DNA Double Strand Breaks with Tip‐Enhanced Raman Scattering, Angewandte Chemie, Volume126, Issue1, which has been published in final form at 10.1002/ange.201307271. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)

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Chemical Sciences not elsewhere classified

Chemical Sciences

Molecular characterization

DNA

Double strand breaks

Tip‐Enhanced Raman Scattering

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