Dietary uptake of a mixture of pp′DDT and four chlorobenzenes from diets with different lipid contents was measured in goldfish (Carassius auratus) in order to investigate the mechanism of intestinal absorption of organic compounds. The results of the experiments suggest that intestinal absorption is basically controlled by chemical diffusion rather than lipid coassimilation. The extent of dietary uptake as indicated by biomagnification factor was strongly correlated with the chemical logKow, indicating that uptake of the chemicals from the gastrointestinal fluid is similar to the uptake from other aqueous environments and lipid content of the food in the range used in these experiments (2.9–10.9%) could not influence the uptake of lipophilic chemicals.