Hydrophilic titanium surface-induced macrophage modulation promotes pro-osteogenic signalling

No Thumbnail Available
File version
Author(s)
Hamlet, Stephen M
Lee, Ryan SB
Moon, Ho-Jin
Alfarsi, Mohammed A
Ivanovski, Saso
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
2019
Size
File type(s)
Location
License
Abstract

Objectives: As biomaterial-induced modulation of mediators of the immune response may be a potential therapeutic approach to enhance wound healing events, the aim of this study was to delineate the effects of titanium surface modification on macrophage phenotype and function. Material and methods: Rodent bone marrow-derived macrophages were polarized into M1 and M2 phenotypes and cultured on micro-rough (SLA) and hydrophilic modified SLA (modSLA) titanium discs. Macrophage phenotype and cytokine secretion were subsequently assessed by immunostaining and ELISA, respectively. Osteoblast gene expression in response to culture in the M1 and M2 macrophage conditioned media was also evaluated over 7 days by RT-PCR. Results: M1 macrophage culture on the modSLA surface promoted an M2-like phenotype as demonstrated by marked CD163 protein expression, Arg1 gene expression and the secretion of cytokines that significantly upregulated in osteoblasts the expression of genes associated with the TGF-ß/BMP signalling pathway and osteogenesis. In comparison, M2 macrophage culture on SLA surface promoted an inflammatory phenotype and cytokine profile that was not conducive for osteogenic gene expression. Conclusions: Macrophages are able to alter or switch their phenotype according to the signals received from the biomaterial surface. A hydrophilic micro-rough titanium surface topography elicits a macrophage phenotype associated with reduced inflammation and enhanced pro-osteogenic signalling.

Journal Title

Clinical Oral Implants Research

Conference Title
Book Title
Edition
Volume
Issue
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)

NHMRC

Grant identifier(s)

APP1036625

Rights Statement
Rights Statement
Item Access Status
Note

This publication has been entered into Griffith Research Online as an Advanced Online Version.

Access the data
Related item(s)
Subject

Biomedical engineering

Dentistry

Clinical sciences

Science & Technology

Life Sciences & Biomedicine

Technology

Dentistry, Oral Surgery & Medicine

Engineering, Biomedical

Persistent link to this record
Citation

Hamlet, SM; Lee, RSB; Moon, H-J; Alfarsi, MA; Ivanovski, S, Hydrophilic titanium surface-induced macrophage modulation promotes pro-osteogenic signalling, Clinical Oral Implants Research, 2019

Collections