A comparison of ABAcard® Hematrace® and RSIDTM-Blood tests on dried, diluted bloodstains treated with leucocrystal violet or luminol

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Streeting, Carl A
Chaseling, Janet
Krosch, Matt N
Wright, Kirsty
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2020
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Abstract

Presumptive tests for blood, such as luminol and leucocrystal violet (LCV), are often used by forensic officers when screening for latent (non-visible) or diluted bloodstains at crime scenes. Where positive reactions are observed, a confirmatory test for the presence of blood may be implemented. This study aimed to compare the efficacy of two such confirmatory tests (ABAcard® Hematrace® and RSIDTM-Blood) on dried, diluted bloodstains that were enhanced using either the Grodsky luminol formulation or LCV. Eighteen replicates per dilution (1:10, 1:100), enhancement, and confirmatory test were performed (144 bloodstains). The RSIDTM-Blood test produced false negative results for all luminol-enhanced bloodstains, regardless of dilution. This test performed slightly better for bloodstains enhanced with LCV, returning approximately 50% positive results. In contrast, the ABAcard® Hematrace® test performed well, returning positive detections for all luminol-treated bloodstains, and all but two LCV-enhanced stains (both 1:100 dilution). Significant differences were observed between the test results and suggested a potential inhibitory effect on the RSIDTM-Blood test from the Grodsky luminol formulation and, to a lesser extent, LCV. This research has demonstrated that the RSIDTM-Blood test is not a reliable confirmatory test faint or latent bloodstains enhanced with luminol or LCV.

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Australian Journal of Forensic Sciences
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This publication has been entered in Griffith Research Online as an advanced online version.
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Forensic biology
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Life Sciences & Biomedicine
Medicine, Legal
Legal Medicine
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Streeting, CA; Chaseling, J; Krosch, MN; Wright, K, A comparison of ABAcard® Hematrace® and RSIDTM-Blood tests on dried, diluted bloodstains treated with leucocrystal violet or luminol, Australian Journal of Forensic Sciences, 2020
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