A peptide-centric approach to breast cancer biomarker discovery utilizing label-free multiple reaction monitoring mass spectrometry

No Thumbnail Available
File version
Author(s)
Metodieva, Gergana
Greenwood, Christina
Alldridge, Louise
Sauven, Paul
Metodiev, Metodi
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)

Michael J Dunn

Date
2009
Size
File type(s)
Location
License
Abstract

We report an approach for multiplex analysis of cancer biomarkers based on the measurement of diagnostic peptides in whole tissue protein digests. Label-free quantitation with MS3 multiple reaction monitoring (MRM) was developed to afford accurate analysis of prospective marker peptides in a panel of breast tumors. This approach provides an economical and robust alternative to stable isotope-based methods. It is equally applicable to the analysis of samples derived from tissue biopsy, aspirate, or plasma and can be easily translated to clinic.

Journal Title

Proteomics - Clinical Applications

Conference Title
Book Title
Edition
Volume

3

Issue

1

Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
Item Access Status
Note
Access the data
Related item(s)
Subject

Proteomics and Intermolecular Interactions (excl. Medical Proteomics)

Medical Biochemistry and Metabolomics

Persistent link to this record
Citation
Collections