Probiotic Screening and Safety Evaluation of Lactobacillus Strains from Plants, Artisanal Goat Cheese, Human Stools, and Breast Milk

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Gotteland, Martin
Jose Cires, Maria
Carvallo, Claudia
Vega, Natalia
Antonieta Ramirez, Maria
Morales, Pamela
Rivas, Patricia
Astudillo, Fernanda
Navarrete, Paola
Dubos, Celine
Figueroa, Alvaro
Troncoso, Miriam
Ulloa, Carolina
Carrasco-Pozo, Catalina
et al.
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2014
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Abstract

The aim of this study was to select autochthonous strains of Lactobacillus from stools of healthy infants and adults, human milk, artisanal goat cheese, and fruits and vegetables according to their probiotic properties and safety. From 421 strains of Lactobacillus isolated, 102 (24.2%) were shown to be tolerant to gastric pH and bile salts; they were used to determine their anti-Helicobacter pylori (agar diffusion assay), antioxidant (oxygen radical absorption capacity), and anti-inflammatory (inhibition of interleukin-8 release by tumor necrosis factor-α-stimulated HT-29 cells) activities as well as their ability to adhere to intestinal (Caco-2) and gastric (AGS) epithelial cells. Results obtained were compared with three commercial probiotic Lactobacillus rhamnosus GG, L. plantarum 299v, and L. johnsonii NCC533. The five strains most efficient according to these activities were subsequently identified by sequencing their 16S rRNA gene, their susceptibility to antibiotics was determined, and their safety evaluated in mice. One strain of L. plantarum was discarded due to the higher prevalence of liver bacterial translocation observed in the animals fed this strain. In conclusion, four autochthonous strains of L. rhamnosus were finally selected with probiotic properties and safety allowing their eventual use in human studies. These results contribute to increase the diversity of probiotic strains available for the development of nutraceuticals and functional foods.

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Journal of Medicinal Food

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17

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4

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This is a copy of an article published in the Journal of Computational Biology. Copyright 2014 Mary Ann Liebert, Inc. Final publication is available from Mary Ann Liebert, Inc., publishers https://doi.org/10.1089/jmf.2013.0030

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Food sciences

Nutrition and dietetics

Science & Technology

Life Sciences & Biomedicine

Chemistry, Medicinal

Food Science & Technology

Nutrition & Dietetics

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Gotteland, M; Jose Cires, M; Carvallo, C; Vega, N; Antonieta Ramirez, M; Morales, P; Rivas, P; Astudillo, F; Navarrete, P; Dubos, C; Figueroa, A; Troncoso, M; Ulloa, C; Luisa Mizgier, M; Carrasco-Pozo, C; Speisky, H; Brunser, O; Figueroa, G, Probiotic Screening and Safety Evaluation of Lactobacillus Strains from Plants, Artisanal Goat Cheese, Human Stools, and Breast Milk, Journal of Medicinal Food, 2014, 17 (4), pp. 487-495

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