Isolation and Characterisation of Novel Lectins from Australian Macrofungi
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Tiralongo, Joe
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Grice, Darren
Tiralongo, Evelin
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Abstract
This thesis describes the isolation and characterisation of novel lectins from Australian indigenous mushroom species and evaluation of their antiproliferative activity against human colon adenocarcinoma (HT29) cell line. In this project, eighteen (18) Australian mushrooms were selected for screening of lectin activity based on published data, availability, distribution, native or exotic, and were collected from the Royal Botanical Garden, Melbourne. The results of the project identified eight (08) mushrooms with lectin activity that led to the isolation and purification of three (03) lectins from three mushroom species. Of these three lectins isolated, two lectins those of, Phlebopus marginatus (PML) and Phaeolus schweinitzii (PSL-2), are novel and one lectin was isolated for the first time from Psathyrella asperospora (PAL). All mushroom species were homogenated with PBS and then were screened for hemagglutination and lectin activity. Hemagglutination activity was evaluated using both neuraminidase treated and untreated rabbit and human A, B, and O erythrocytes. Lectin activity was determined by the ability of various mono- and di-saccharides to inhibit hemagglutination activity. The results of this screening study identified eight (08) mushroom species that showed lectin activity, Agaricus bitorquis, Chlorophyllum brunneum, Coprinus comatus, Cortinarius sp. TWM 1710, Omphalotus nidiformis, Phlebopus marginatus, Psathyrella asperospora and Phaeolus schweinitzii. The remaining ten (10) mushroom species only showed hemagglutination activity devoid of any lectin activity.
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Thesis (PhD Doctorate)
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Doctor of Philosophy (PhD)
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Institute for Glycomics
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The author owns the copyright in this thesis, unless stated otherwise.
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Subject
Macrofungi
Novel lectins
Human colon adenocarcinoma (HT29) cell line
Phlebopus marginatus (PML)
Phaeolus schweinitzii (PSL-2)