Poly(amino acids) as a potent self-adjuvanting delivery system for peptide-based nanovaccines

Loading...
Thumbnail Image
File version

Version of Record (VoR)

Author(s)
Skwarczynski, Mariusz
Zhao, Guangzu
Boer, Jennifer
Ozberk, Victoria
Azuar, Armira
Cruz, Jazmina Gonzalez
Giddam, Ashwini Kumar
Khalil, Zeinab
Pandey, Manisha
Shibu, Mohini A
Hussein, Waleed M
Nevagi, Reshma J
Batzloff, Michael R
Wells, James
Capon, Robert J
Plebanski, Magdalena
Good, Michael F
Toth, Istvan
Primary Supervisor
Other Supervisors
Editor(s)
Date
2020
Size
File type(s)
Location
Abstract

To be optimally effective, peptide-based vaccines need to be administered with adjuvants. Many currently available adjuvants are toxic, not biodegradable; they invariably invoke adverse reactions, including allergic responses and excessive inflammation. A nontoxic, biodegradable, biocompatible, self-adjuvanting vaccine delivery system is urgently needed. Herein, we report a potent vaccine delivery system fulfilling the above requirements. A peptide antigen was coupled with poly-hydrophobic amino acid sequences serving as self-adjuvanting moieties using solid-phase synthesis, to produce fully defined single molecular entities. Under aqueous conditions, these molecules self-assembled into distinct nanoparticles and chain-like aggregates. Following subcutaneous immunization in mice, these particles successfully induced opsonic epitope-specific antibodies without the need of external adjuvant. Mice immunized with entities bearing 15 leucine residues were able to clear bacterial load from target organs without triggering the release of soluble inflammatory mediators. Thus, we have developed a well-defined and effective self-adjuvanting delivery system for peptide antigens.

Journal Title

Science Advances

Conference Title
Book Title
Edition
Volume

6

Issue

5

Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement

© 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

Item Access Status
Note
Access the data
Related item(s)
Subject

Immunology

Clinical sciences

Science & Technology

Multidisciplinary Sciences

Science & Technology - Other Topics

NLRP3 INFLAMMASOME

VACCINE

Persistent link to this record
Citation

Skwarczynski, M; Zhao, G; Boer, J; Ozberk, V; Azuar, A; Cruz, JG; Giddam, AK; Khalil, Z; Pandey, M; Shibu, MA; Hussein, WM; Nevagi, RJ; Batzloff, MR; Wells, J; Capon, RJ; Plebanski, M; Good, MF; Toth, I, Poly(amino acids) as a potent self-adjuvanting delivery system for peptide-based nanovaccines, Science Advances, 2020, 6 (5)

Collections