Moraxella catarrhalis NucM is an entry nuclease involved in extracellular DNA and RNA degradation, cell competence and biofilm scaffolding

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Tan, Aimee
Li, Wing-Sze
Verderosa, Anthony D
Blakeway, Luke
Mubaiwa, Tsitsi D
Totsika, Makrina
Seib, Kate L
Griffith University Author(s)
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2019
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Abstract

Moraxella catarrhalis is a host-adapted bacterial pathogen that causes otitis media and exacerbations of chronic obstructive pulmonary disease. This study characterises the conserved M. catarrhalis extracellular nuclease, a member of the ββα metal finger family of nucleases, that we have named NucM. NucM shares conserved sequence motifs from the ββα nuclease family, including the DRGH catalytic core and Mg2+ co-ordination site, but otherwise shares little primary sequence identity with other family members, such as the Serratia Nuc and pneumococcal EndA nucleases. NucM is secreted from the cell and digests linear and circular nucleic acid. However, it appears that a proportion of NucM is also associated with the cell membrane and acts as an entry nuclease, facilitating transformation of M. catarrhalis cells. This is the first example of a ββα nuclease in a Gram negative bacteria that acts as an entry nuclease. In addition to its role in competence, NucM affects cell aggregation and biofilm formation by M. catarrhalis, with ΔnucM mutants having increased biofilm biomass. NucM is likely to increase the ability of cells to survive and persist in vivo, increasing the virulence of M. catarrhalis and potentially affecting the behaviour of other pathogens that co-colonise the otorhinolaryngological niche.

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Scientific Reports

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9

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1

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NHMRC

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APP1099279

APP1045235

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© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Bacteriology

Proteomics and intermolecular interactions (excl. medical proteomics)

Science & Technology

Multidisciplinary Sciences

Science & Technology - Other Topics

STREPTOCOCCUS-PNEUMONIAE

HAEMOPHILUS-INFLUENZAE

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Tan, A; Li, W-S; Verderosa, AD; Blakeway, L; Mubaiwa, TD; Totsika, M; Seib, KL, Moraxella catarrhalis NucM is an entry nuclease involved in extracellular DNA and RNA degradation, cell competence and biofilm scaffolding, Scientific Reports, 2019, 9 (1)

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