Reliable cell purification and determination of cell purity: crucial aspects of olfactory ensheathing cell transplantation for spinal cord repair

Loading...
Thumbnail Image
File version

Version of Record (VoR)

Author(s)
Reshamwala, Ronak
Shah, Megha
Belt, Lucy
Ekberg, Jenny AK
St John, James A
Primary Supervisor
Other Supervisors
Editor(s)
Date
2020
Size
File type(s)
Location
Abstract

Transplantation of olfactory ensheathing cells, the glia of the primary olfactory nervous system, has been trialed for spinal cord injury repair with promising but variable outcomes in animals and humans. Olfactory ensheathing cells can be harvested either from the lamina propria beneath the neuroepithelium in the nasal cavity, or from the olfactory bulb in the brain. As these areas contain several other cell types, isolating and purifying olfactory ensheathing cells is a critical part of the process. It is largely unknown how contaminating cells such as fibroblasts, other glial cell types and supporting cells affect olfactory ensheathing cell function post-transplantation; these cells may also cause unwanted side-effects. It is also, however, possible that the presence of some of the contaminant cells can improve outcomes. Here, we reviewed the last decade of olfactory ensheathing cell transplantation studies in rodents, with a focus on olfactory ensheathing cell purity. We analyzed how purification methods and resultant cell purity differed between olfactory mucosa- and olfactory bulb-derived cell preparations. We analyzed how the studies reported on olfactory ensheathing cell purity and which criteria were used to define cells as olfactory ensheathing cells. Finally, we analyzed the correlation between cell purity and transplantation outcomes. We found that olfactory bulb-derived olfactory ensheathing cell preparations are typically purer than mucosa-derived preparations. We concluded that there is an association between high olfactory ensheathing cell purity and favourable outcomes, but the lack of olfactory ensheathing cell-specific markers severely hampers the field.

Journal Title

Neural Regeneration Research

Conference Title
Book Title
Edition
Volume

15

Issue

11

Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement

© The Author(s) 2020. This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License (https://creativecommons.org/licenses/by-nc-sa/3.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the original work is cited properly.

Item Access Status
Note
Access the data
Related item(s)
Subject

Biological sciences

Neurosciences

Science & Technology

Life Sciences & Biomedicine

Cell Biology

Neurology

Persistent link to this record
Citation

Reshamwala, R; Shah, M; Belt, L; Ekberg, JAK; St John, JA, Reliable cell purification and determination of cell purity: crucial aspects of olfactory ensheathing cell transplantation for spinal cord repair, Neural Regeneration Research, 2020, 15 (11), pp. 2016-2026

Collections