A p-menth-1-ene-4,7-diol (EC-1) from eucalyptus camaldulensis dhnh. triggers apoptosis and cell cycle changes in ehrlich ascites carcinoma cells
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Khanam, Jahan Ara
Khatun, Mahbuba
Zuberi, Natasha
Khatun, Laboni
Kabir, Syed Rashel
Abu Reza, Md
Ali, MM
Rabbi, MA
Gopalan, Vinod
Lam, Alfred King-Yin
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Abstract
Anticancer activities of p-menth-1-ene-4,7-diol (EC-1) isolated from Eucalyptus camaldulensis Dhnh. were studied on Ehrlich ascites carcinoma (EAC) cells by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. Anticancer activities also analyzed in EAC-bearing mice by assessment of cancer growth inhibition, changes in cancer volume, changes in life span, and hematological parameters. Apoptosis was analyzed by fluorescence microscope, DNA fragmentation assay, and flow cytometry. The expression of apoptosis-related genes, Bcl-2, Bcl-X, PARP-1, p53, and Bax, were analyzed using polymerase chain reaction (PCR). EC-1 significantly inhibited proliferation of EAC cells in vivo and restored the altered hematological parameters of EAC-bearing mice. Cytological observation by fluorescence microscope showed apoptosis of EAC cells upon treatment with EC-1. Also, DNA fragmentation assay revealed EAC cells' apoptosis following EC-1 treatment. Increased mRNA expressions of p53 and Bax genes and negative expressions of Bcl-2 and Bcl-X were observed in cells treated with EC-1. These findings confirmed the induction of apoptosis by EC-1. In addition, MTT assay showed dose-dependent anticancer activity of EC-1 against EAC cell. Cell cycle analysis revealed that EC-1 treatment caused suppression of EAC cells at S phase. To conclude, EC-1 is a novel anticancer compound and showed antiproliferative and apoptotic activities in cellular and mice models.
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Phytotherapy Research
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29
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4
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© 2015 John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: A p-menth-1-ene-4,7-diol (EC-1) from eucalyptus camaldulensis dhnh. triggers apoptosis and cell cycle changes in ehrlich ascites carcinoma cells, Phytotherapy Research, Volume 29, Issue 4, April 2015, Pages 573-581, which has been published in final form at 10.1002/ptr.5288. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
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Chemical sciences
Biological sciences
Biomedical and clinical sciences
Health sciences