Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs
File version
Author(s)
Furuno, M.
Kasukawa, T.
Adachi, J.
Bona, H.
Kondo, S.
Nikaido, I.
Osato, N.
Willming, L.
Saito, R.
Suzuki, H.
Yamanaka, I
Kiyosawa, H.
Yagi, K.
Tomaru, Y.
Hasegawa, H.
Nogami, A.
Schonbach, C.
Gojobori, T.
Baldarelli, R.
Hill, D.
Bult, C.
Hume, D. A.
Quackenbush, J.
Schrimi, L.
Kanapin, A.
Matsuda, H.
Batalov, S.
Beisel, K.
Blake, J.
Bradt, D.
Brusic, V.
Chothia, C.
Corbani, L.
Cousins, S.
Dalla, E.
Dragani, T.
Fletcher, C.
Forrest, A.
Frazer, K.
Gaasterland, T.
Gariboldi, M.
Gissi, C.
Godzik, A.
Gough, J.
Grimmond, S.
Gustincich, S.
Hirokawa, N.
Jackson, I.
Jarvis, E.
Kanai, A.
Kawaji, H.
Kawasawa, Y.
Kedierski, R.
King, B.
Konagaya, A.
Kurochkin, I.
Lee, Y.
Lenhard, B.
Lyons, P.
Maglott, D.
Maltais, L.
Marchionni, L.
McKenzie, L.
Miki, H.
Nagashima, T.
Nagashima, K.
Numata, K.
Okido, T.
Pavan, W.
Pertea, G.
Pesole, G.
Petrovsky, N.
Pillai, R.
Pontius, J.
Qi, D.
Ramachandran, S.
Ravasi, T.
Reed, J.
Reed, D.
Reid, J.
Ring, B.
Ringwald, M.
Sandelin, A.
Schneider, C.
M Semple, C.
Setou, M.
Shimada, K.
Sultana, R.
Wells, C.
Takenaka, Y.
Taylor, M.
Teasdale, R.
Tomita, M.
Verardo, R.
Wagner, L.
Wahlestedt, C.
Wang, Y.
Watanabe, Y.
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
Size
File type(s)
Location
License
Abstract
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the mouse, and to ensure that each is represented in a physical collection of clones. Here we report the manual annotation of 60,770 full-length mouse complementary DNA sequences. These are clustered into 33,409 'transcriptional units', contributing 90.1% of a newly established mouse transcriptome database. Of these transcriptional units, 4,258 are new protein-coding and 11,665 are new non-coding messages, indicating that non-coding RNA is a major component of the transcriptome. 41% of all transcriptional units showed evidence of alternative splicing. In protein-coding transcripts, 79% of splice variations altered the protein product. Whole-transcriptome analyses resulted in the identification of 2,431 sense-antisense pairs. The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics
Journal Title
Nature
Conference Title
Book Title
Edition
Volume
420
Issue
6915