The spread of antibiotic resistant bacteria is a growing problem worldwide. Staphylococci bacteria is recognized as a genus of bacteria often closely related to human and animal hosts. Macaques are one type of non-human primate host that could potentially spread antibiotic resistant bacteria in an environment in close proximity to humans. Bacteria isolated from locations in Brunei Darussalam with and without the presence of long-tailed macaques revealed the existence of multiple drug resistant staphylococci bacteria with a higher prevalence observed in locations with a presence of long-tailed macaques. These findings have important zoonotic implications on infectious disease control and surveillance in public spaces frequented by macaques.