Neuroprotective effects of a standardized flavonoid extract from Diospyros kaki leaves

No Thumbnail Available
File version
Author(s)
Bei, Weijian
Zang, Linquan
Guo, Jiao
Peng, Wenlie
Xu, Anlong
Good, David A
Hu, Yinming
Wu, Wei
Hu, Dehui
Zhu, Xinghong
Wei, Ming
Li, Chuyuan
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
2009
Size
File type(s)
Location
License
Abstract

Ethnopharmacological relevance: Flavonoids, extracted from the leaves of Diospyros kaki, are the main therapeutic components of NaoXingQing (NXQ), a potent and patented Chinese herbal remedy widely used in China for the treatment of apoplexy syndrome. Aim of the study: To investigate the neuroprotective effects of FLDK-P70, a standardized flavonoid extract, using in vivo rat models of both focal ischemia/reperfusion (I/R) injury induced by middle cerebral artery occlusion (MCAO) and on transient global brain ischemia induced by four-vessel occlusion (4-VO). We also aim to examine the effects of FLDK-P70 on glutamate-induced cell injury of hippocampal neurons as well as on hypoxia-induced injury of cortical neurons in primary cell culture. Materials and methods and results: Administration of FLDK-P70 for 12 days (40, 80 mg/kg body weight, p.o., 5 days before and 7 days after 4-VO) increased the survival of hippocampal CA1 pyramidal neurons after transient global brain ischemia. Similarly, administration of FLDK-P70 for 7 days (40, 80 mg/kg body weight, p.o., 3 days before and 4 days after MCAO) significantly reduced the lesion of the insulted brain hemisphere and improved the neurological behavior of rats. In primary rat hippocampal neuronal cultures, pretreatment with FLDK-P70 (5, 10 g/ml) protected neurons from glutamate-induced excitotoxic neuronal death in a dose-dependent manner. In primary rat cerebral cortical neuronal culture, pretreatment with FLDK-P70 (25, 100 g/ml) also reduced hypoxia-reoxygen induced neuronal death and apoptosis in a dose-dependent manner. Conclusions: These in vivo and in vitro results suggest that FLDK-P70 significantly protects rats fromMCAO and 4-VO ischemic injury in vivo and protects hippocampal neurons from glutamate-induced excitotoxic injury as well as cortical neurons from hypoxia-induced injury in vitro. The mechanisms of these effects may be due to the antioxidative activity of the flavonoids. These results convincingly demonstrate that FLDK-P70 may be useful for the prevention and treatment of ischemia/reperfusion injury and other related neurodegenerative diseases.

Journal Title

Journal of Ethnopharmacology

Conference Title
Book Title
Edition
Volume

126

Issue
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
Item Access Status
Note
Access the data
Related item(s)
Subject

Plant biology

Traditional, complementary and integrative medicine

Traditional Chinese medicine and treatments

Pharmacology and pharmaceutical sciences

Persistent link to this record
Citation
Collections