Determination of novel biomarkers and pathways shared by colorectal cancer and endometrial cancer via comprehensive bioinformatics analysis

Thumbnail Image
File version
Version of Record (VoR)
Author(s)
Rahman, F
Mahmud, P
Karim, R
Hossain, T
Islam, F
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
2020
Size
File type(s)
Location
License
https://creativecommons.org/licenses/by-nc-nd/4.0/
Abstract

Background: Endometrial cancer (EC) is a common female genital tract malignancy of women, and colorectal cancer (CRC) is one of its risk factors. However, the mutual genes and molecular pathways shared by these two diseases have not yet been ascertained. Objectives: In the present study, we aimed to reveal candidate biomarkers and molecular interactions between CRC and EC to understand the common disease mechanism. Materials and methods: We performed differential analysis of CRC and EC microarray data by employing limma to reveal differentially expressed genes (DEGs). Then, we used mutual DEGs between these diseases to obtain significant biological processes and pathways by performing enrichment analyses. In addition, to reveal candidate biomarkers and regulatory transcripts, we analyzed different networks using bioinformatics tools. Results: We identified 286 overlapped DEGs between CRC and EC transcriptomes datasets. Enrichment analysis of these aberrantly expressed genes revealed that they were significantly enriched in cancer pathways signaling. Based on topological analysis of the PPI network, we revealed 11 hub proteins including JUN, MYC, FOS, EGR1, LEF1, CDC42, CTGF, ADAM10, CYR61, FOXA1, and UBE2I. We also identified seven significant transcription factors (TFs) namely FOXC1, GATA2, YY1, E2F1, CREB1, HINFP, and FOXL1 and five miRNAs including hsa-miR-124-3p, hsa-miR-106b-5p, hsa-miR-501-3p, hsa-miR-29b-3p, and hsa-miR-145-5p that regulate expressions of the DEGs. Conclusion: We have identified novel biomarkers and molecular pathways shared by CRC and EC that will help to understand underlying common molecular mechanisms and to develop potential therapeutics.

Journal Title
Informatics in Medicine Unlocked
Conference Title
Book Title
Edition
Volume
20
Issue
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
© 2020 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
Item Access Status
Note
Access the data
Related item(s)
Subject
Biological sciences
Health services and systems
Persistent link to this record
Citation
Rahman, F; Mahmud, P; Karim, R; Hossain, T; Islam, F, Determination of novel biomarkers and pathways shared by colorectal cancer and endometrial cancer via comprehensive bioinformatics analysis, Informatics in Medicine Unlocked, 2020, 20, pp. 100376
Collections