Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

No Thumbnail Available
File version
Author(s)
Pearen, Michael A
Lim, Hong Kiat
Gratte, Francis D
Fernandez-Rojo, Manuel A
Nawaratna, Sujeevi K
Gobert, Geoffrey N
Olynyk, John K
Tirnitz-Parker, Janina EE
Ramm, Grant A
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
2020
Size
File type(s)
Location
License
Abstract

Understanding the mechanisms of liver injury, hepatic fibrosis, and cirrhosis that underlie chronic liver diseases (i.e., viral hepatitis, non-alcoholic fatty liver disease, metabolic liver disease, and liver cancer) requires experimental manipulation of animal models and in vitro cell cultures. Both techniques have limitations, such as the requirement of large numbers of animals for in vivo manipulation. However, in vitro cell cultures do not reproduce the structure and function of the multicellular hepatic environment. The use of precision-cut liver slices is a technique in which uniform slices of viable mouse liver are maintained in laboratory tissue culture for experimental manipulation. This technique occupies an experimental niche that exists between animal studies and in vitro cell culture methods. The presented protocol describes a straightforward and reliable method to isolate and culture precision-cut liver slices from mice. As an application of this technique, ex vivo liver slices are treated with bile acids to simulate cholestatic liver injury and ultimately assess the mechanisms of hepatic fibrogenesis.

Journal Title
Journal of Visualized Experiments (JoVE)
Conference Title
Book Title
Edition
Volume
2020
Issue
157
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
Item Access Status
Note
Access the data
Related item(s)
Subject
Biochemistry and cell biology
Psychology
Cognitive and computational psychology
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
Medicine
Issue 157
Persistent link to this record
Citation
Pearen, MA; Lim, HK; Gratte, FD; Fernandez-Rojo, MA; Nawaratna, SK; Gobert, GN; Olynyk, JK; Tirnitz-Parker, JEE; Ramm, GA, Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology, Journal of Visualized Experiments (JoVE), 2020, 2020 (157)
Collections