The purpose of this study was to investigate the contribution of per- and polyfluoroalkyl substances (PFASs) to groundwater at a location where recycled water from a wastewater treatment plant (WWTP) is used to irrigate crops. Groundwater from Werribee South, located west of Melbourne, Australia, was sampled over two campaigns in 2017 and 2018, extracted using solid phase extraction (SPE) and analysed with liquid chromatography-tandem mass spectrometry (LC-MS/MS-QQQ). PFASs were detected in 100% of the groundwater samples. The sum total of twenty PFAS compounds (∑ 20 PFASs) for all sites in the study ranged from <0.03 to 74 ng/L (n = 28) and the highest levels of which were observed in the centre of the irrigation district. Perfluorooctanesulfonic acid (PFOS) was the most detected compound overall (96%) with a mean concentration of 11 ng/L (<0.03–34 ng/L), followed by perfluorobutanesulfonic acid (PFBS; 86%, 4.4 ng/L), perfluorooctanoic acid (PFOA; 82%, 2.2 ng/L) and perfluorobutanoic acid (PFBA; 77%, 6.1 ng/L). Concentrations of PFASs found in this study are greater than background levels of PFASs detected in groundwater and are in the range of concentrations typically detected in wastewater effluent. This study presents evidence that the use of recycled water can be a source of PFAS contamination to groundwater.