NT-ProBNP Levels in Saliva and Its Clinical Relevance to Heart Failure

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Foo, Jared Yong Yang
Wan, Yunxia
Kostner, Karam
Arivalagan, Alicia
Atherton, John
Cooper-White, Justin
Dimeski, Goce
Punyadeera, Chamindie
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2012
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https://creativecommons.org/licenses/by/4.0/
Abstract

Background: Current blood based diagnostic assays to detect heart failure (HF) have large intra-individual and inter-individual variations which have made it difficult to determine whether the changes in the analyte levels reflect an actual change in disease activity. Human saliva mirrors the body's health and well being and ~20% of proteins that are present in blood are also found in saliva. Saliva has numerous advantages over blood as a diagnostic fluid which allows for a non-invasive, simple, and safe sample collection. The aim of our study was to develop an immunoassay to detect NT-proBNP in saliva and to determine if there is a correlation with blood levels. Methods: Saliva samples were collected from healthy volunteers (n = 40) who had no underlying heart conditions and HF patients (n = 45) at rest. Samples were stored at -80°C until analysis. A customised homogeneous sandwich AlphaLISA(R) immunoassay was used to quantify NT-proBNP levels in saliva. Results: Our NT-proBNP immunoassay was validated against a commercial Roche assay on plasma samples collected from HF patients (n = 37) and the correlation was r2 = 0.78 (p<0.01, y = 1.705× +1910.8). The median salivary NT-proBNP levels in the healthy and HF participants were <16 pg/mL and 76.8 pg/mL, respectively. The salivary NT-proBNP immunoassay showed a clinical sensitivity of 82.2% and specificity of 100%, positive predictive value of 100% and negative predictive value of 83.3%, with an overall diagnostic accuracy of 90.6%. Conclusion: We have firstly demonstrated that NT-proBNP can be detected in saliva and that the levels were higher in heart failure patients compared with healthy control subjects. Further studies will be needed to demonstrate the clinical relevance of salivary NT-proBNP in unselected, previously undiagnosed populations. © 2012 Foo et al.

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PLoS One
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7
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10
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© 2012 Foo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
BRAIN NATRIURETIC PEPTIDE
DIAGNOSTIC-ACCURACY
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Foo, JYY; Wan, Y; Kostner, K; Arivalagan, A; Atherton, J; Cooper-White, J; Dimeski, G; Punyadeera, C, NT-ProBNP Levels in Saliva and Its Clinical Relevance to Heart Failure, PLoS One, 2012, 7 (10), pp. e48452
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