Deregulation of miR-126 expression in colorectal cancer pathogenesis and its clinical significance
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Gopalan, Vinod
Wahab, Riajul
Lu, Cu-Tai
Smith, Robert Anthony
Lam, Alfred King-Yin
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Abstract
In this study, we investigated the expression profiles and clinicopathological significance of miR-126 in large cohort of patients with colorectal cancers as well the cellular repercussions of miR-126 in colon cancer cells along with its targets in-vitro. Down regulation of miR-126 expression was associated with histological subtypes, peri-neural tumour infiltration, microsatellite instability and pathological staging of colorectal cancers (p<0.05). Low miR-126 expression was also associated with poorer survival in patients with colorectal cancer. Analysis of matched tissues from the same patient revealed that approximately 70% of the tested patients had similar levels of expression of miR-126 in primary cancer and cancer metastases in both lymph node and distant metastases. In addition, induced overexpression of miR-126 showed reduced cell proliferation, increased apoptosis and decreased accumulation of cells in the G0–G1 phase of the colon cancer cells. Furthermore, SW480+miR-126 cells showed reduced BCL-2 and increased P53 protein expression. To conclude, deregulation of miR-126 in colorectal cancer at the tissue and cellular levels as well as its correlation with various clinicopathological parameters confirm the cancer suppressive role of miR-126 in colorectal cancer.
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Experimental Cell Research
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339
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2
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Biochemistry and cell biology
Biochemistry and cell biology not elsewhere classified
Clinical sciences