A Comparison of Two Methods for the Detection of Circulating Tumour Cells in Patients with Oral Cavity Cancer
File version
Accepted Manuscript (AM)
Author(s)
Wong, Gordon
Wang, Weilan
Thomson, Peter
Lam, Alfred K
Choi, Siu-Wai
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
Size
File type(s)
Location
License
Abstract
BACKGROUND: Circulating tumour cells (CTCs) detected in patient blood samples are relevant as diagnostic and prognostic markers offering insights into tumour behaviour and guiding treatment of cancer at an individualised level. The aim of this study is to ascertain the feasibility of detecting CTCs in oral squamous cell carcinoma (OSCC) using two different methods so as to determine the optimal method for the study of this cancer. METHODS: Comparison of the numbers of CTCs, circulating tumour micro-emboli (CTMs) and circulating tumour endothelial cells (CTECs) was undertaken in forty clinical samples of oral squamous cell carcinoma (OSCC) determined by filtration (ISET®) ) and in-situ fluorescent immunostaining (i-FISH, Cytelligen®) immunostaining and in-situ hybridisation. RESULTS: i-FISH detected CTCs in 80% of samples compared to 40% of samples analysed by microfiltration. i-FISH detected CTCs in a further 40% of samples in which microfiltration did not detect CTCs. No CTC clusters were detected by microfiltration while i-FISH detected CTM in 12.5% of samples. i-FISH analysis detected CTECs in 20/40 samples. CONCLUSION: These results highlight significant differences in detection of CTCs, CTM and CTECs between i-FISH and microfiltration when applied to OSCC samples, suggesting that technologies capable of detecting circulating aneuploidic cells more accurately detect CTCs. i-FISH also detected CTM and CTEC not detected using ISET®. With proven prognostic relevance in adenocarcinomas, accurate enumeration of CTCs, CTMs and CTECs may be a clinically useful tool in the management of OSCC and may aid in the reduction of false negative diagnoses.
Journal Title
Journal of Oral Pathology & Medicine
Conference Title
Book Title
Edition
Volume
Issue
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
© 2021 John Wiley & Sons A/S. This is the peer reviewed version of the following article: A Comparison of Two Methods for the Detection of Circulating Tumour Cells in Patients with Oral Cavity Cancer, Journal of Oral Pathology & Medicine, 2021, which has been published in final form at https://doi.org/10.1111/jop.13240. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
Item Access Status
Note
This publication has been entered in Griffith Research Online as an advanced online version.
Access the data
Related item(s)
Subject
Clinical sciences
Oncology and carcinogenesis
Dentistry
Fluorescence In Situ Hybridization
ISET
circulating tumour cells
oral cancer
Persistent link to this record
Citation
Curtin, J; Wong, G; Wang, W; Thomson, P; Lam, AK; Choi, S-W, A Comparison of Two Methods for the Detection of Circulating Tumour Cells in Patients with Oral Cavity Cancer, Journal of Oral Pathology & Medicine, 2021