Effect of Coffee Combining Green Coffee Bean Constituents with Typical Roasting Products on the Nrf2/ARE Pathway in Vitro and in Vivo

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Volz, Nadine
Boettler, Ute
Winkler, Swantje
Teller, Nicole
Schwarz, Chrisoph
Bakuradze, Tamara
Eizenbrand, Gerhard
Haupt, Larisa
Griffiths, Lyn
Stiebitz, Herbert
Bytof, Gerhard
Lantz, Ingo
Lang, Roman
Hofmann, Thomas
Somoza, Veronika
Marko, Doris
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2012
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Abstract

This study investigated Nrf2-activating properties of a coffee blend combining raw coffee bean constituents with 5-O-caffeoylquinic acid (CGA) as a lead component with typical roasting products such as N-methylpyridinium (NMP). In cell culture (HT29) the respective coffee extract (CN-CE) increased nuclear Nrf2 translocation and enhanced the transcription of ARE-dependent genes as exemplified for NAD(P)H:quinone oxidoreductase and glutathione-S-transferase (GST)A1, reflected in the protein level by an increase in GST enzyme activity. In a pilot human intervention study (29 healthy volunteers), daily consumption of 750 mL of CN-coffee for 4 weeks increased Nrf2 transcription in peripheral blood lymphocytes on average. However, the transcriptional response pattern of Nrf2/ARE-dependent genes showed substantial interindividual variations. The presence of SNPs in the Nrf2-promoter, reported recently, as well as the detection of GSTT1*0 (null) genotypes in the study collective strengthens the hypothesis that coffee acts as a modulator of Nrf2-dependent gene response in humans, but genetic polymorphisms play an important role in the individual response pattern.

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Journal of Agricultural and Food Chemistry

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60

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38

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Medical and Health Sciences not elsewhere classified

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Agricultural and Veterinary Sciences

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