A range of chitosan–based biomaterials have recently been used to perform sutureless, laser‐activated tissue repair. Laser‐activation has the advantage of bonding to tissue through a non‐contact, aseptic mechanism. Chitosan adhesive films have also been shown to adhere to sheep intestine strongly without any chemical modification to chitosan. In this study, we continue to investigate chitosan adhesive films and explore the impact on the tissue repair strength and tensile strength characteristics of four types of adhesive film based on chitosan with different molecular weight and degree of deacetylation. Results showed that adhesives based on chitosan with medium molecular weight achieved the highest bonding strength, tensile strength and E‐modulus when compared to the other adhesives.