Campylobacter jejuni is a major cause of human bacterial gastroenteritis worldwide, however despite its importance, relatively little is known about its mechanisms of pathogenesis or interaction with the host. Previous studies have identified flagella, motility, and other surface structures as essential requirements for C. jejuni pathogenesis, with exact interactions still to be elucidated. It has been hypothesised that C. jejuni interacts with cell surface glycoproteins known as mucins; these structures have previously been identified as chemoattractants for C. jejuni. Specifically, MUC1 is a membrane bound mucin expressed on the cell surface of the epithelium lining the gastric mucosa. This study proposed that C. jejuni interacts with MUC1 to facilitate attachment and penetration into host mucosal cells. To investigate this hypothesis, in vitro co-cultures using an intestinal cell line expressing MUC1 were employed. Co-culture assays showed correlation with previous observations that MUC1 promotes more rapid adherence to the cell surface, and further demonstrated adherence and invasion of C. jejuni 81116 was significantly increased in the presence of MUC1. Additionally, an alternative co-culture assay that uses incubating conditions that resemble the gastrointestinal mucosa more closely was established. These assay conditions produced more reliable and consistent assay data for a microaerophilic organism.