Mass Spectrometry for Biomarkers Discovery in Esophageal Squamous Cell Carcinoma

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Islam, F
Gopalan, V
Lam, AK
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2020
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Abstract

Mass spectrometry-based proteomics analysis could categorize proteins and study their interactions in large scale in human cancers. By this method, many proteins are upregulated or downregulated in esophageal squamous cell carcinoma (ESCC) when compared to nonneoplastic esophageal mucosae. The method can also be used to identify novel, effective biomarkers for early diagnosis or predict prognosis of patients with ESCC. These changes are associated with different clinical and pathological parameters. Different biological matrices such as pathological tissue, body fluids, and cancer cell lines-based proteomics have widely been used. Herein, we described cell line-based label-free shotgun proteomics (in-solution tryptic digestion) to identify the protein biomarkers differently expressed in ESCC.

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Methods in Molecular Biology
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Other chemical sciences
Biochemistry and cell biology
Medicinal and biomolecular chemistry
Biomarkers
Cancer
ESCC
Label-free proteomics
Shotgun proteomics
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Islam, F; Gopalan, V; Lam, AK, Mass Spectrometry for Biomarkers Discovery in Esophageal Squamous Cell Carcinoma, Methods in Molecular Biology, 2020, 2129, pp. 259-268
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