Enhanced Biological Straw Saccharification Through Coculturing of Lignocellulose-Degrading Microorganisms

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Taha, Mohamed
Shahsavari, Esmaeil
Al-Hothaly, Khalid
Mouradov, Aidyn
Smith, Andrew T
Ball, Andrew S
Adetutu, Eric M
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2015
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Abstract

Lignocellulosic waste (LCW) is an abundant, low-cost, and inedible substrate for the induction of lignocellulolytic enzymes for cellulosic bioethanol production using an efficient, environmentally friendly, and economical biological approach. In this study, 30 different lignocellulose-degrading bacterial and 18 fungal isolates were quantitatively screened individually for the saccharification of four different ball-milled straw sub-strates: wheat, rice, sugarcane, and pea straw. Rice and sugarcane straws which had similar Fourier transform-infrared spectroscopy profiles were more degradable, and resulted in more hydrolytic enzyme production than wheat and pea straws. Crude enzyme produced on native straws performed better than those on artificial substrates (such as cellulose and xylan). Four fungal and five bacterial isolates were selected (based on their high strawase activities) for constructing dual and triple microbial combinations to investigate microbial synergistic effects on saccharification. Combinations such as FUNG16-FUNG17 (Neosartorya fischeri–Myceliophthora thermophila) and RMIT10-RMIT11 (Aeromonas hydrophila–Pseudomonas poae) enhanced saccharification (3-and 6.6-folds, respectively) compared with their monocultures indicating the beneficial effects of synergism between those isolates. Dual isolate combinations were more efficient at straw saccharification than triple combinations in both bacterial and fungal assays. Overall, co-culturing can result in significant increases in saccharification which may offer significant commercial potential for the use of microbial consortia.

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Applied Biochemistry and Biotechnology

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175

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8

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© 2015 International Union of Biochemistry and Molecular Biology, Inc. Published by Blackwell Verlag GmbH. This is the peer reviewed version of the following article: Enhanced Biological Straw Saccharification Through Coculturing of Lignocellulose-Degrading Microorganisms, Biotechnology and Applied Biochemistry, April 2015, Volume 175, Issue 8, which has been published in final form at 10.1007%2Fs12010-015-1539-9. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)

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Biological sciences

Microbiology not elsewhere classified

Environmental biotechnology not elsewhere classified

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