Identifying the structures of individual RNA isoforms inside cells
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Mak, Johnson
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Abstract
Nano-DMS-MaP focuses in on the structures of individual RNA isoforms, enabling direct examination of the structural diversity of different RNAs inside cells. A new technique by Bohn and Gribling-Burrer et al. published in this issue combines chemical probing (using dimethyl sulfate, DMS), an ultraprocessive reverse transcriptase and long-read sequencing to obtain isoform-resolved RNA structural information. This approach, termed Nano-DMS-MaP, stands to revolutionize our understanding of RNA structure and function1.
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Nature Methods
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20
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6
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© 2023 Nature Publishing Group. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
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Biochemistry and cell biology
Genetics
Biological sciences
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Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
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Kenyon, JC; Mak, J, Identifying the structures of individual RNA isoforms inside cells, Nature Methods, 2023, 20 (6), pp. 791-792