Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53
File version
Accepted Manuscript (AM)
Author(s)
Tsai, Feng-Ching
Dibsy, Rayane
de Poret, Aurore
Manzi, John
Merida, Peggy
Muller, Remi
Lappalainen, Pekka
Roingeard, Philippe
Mak, Johnson
Bassereau, Patricia
Favard, Cyril
Muriaux, Delphine M
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
Size
File type(s)
Location
Abstract
During HIV-1 particle formation, the requisite plasma membrane curvature is thought to be solely driven by the retroviral Gag protein. Here, we reveal that the cellular I-BAR protein IRSp53 is required for the progression of HIV-1 membrane curvature to complete particle assembly. SiRNA-mediated knockdown of IRSp53 gene expression induces a decrease in viral particle production and a viral bud arrest at half completion. Single molecule localization microscopy at the cell plasma membrane shows a preferential localization of IRSp53 around HIV-1 Gag assembly sites. In addition, we observe the presence of IRSp53 in purified HIV-1 particles. Finally, HIV-1 Gag protein preferentially localizes to curved membranes induced by IRSp53 I-BAR domain on giant unilamellar vesicles. Overall, our data reveal a strong interplay between IRSp53 I-BAR and Gag at membranes during virus assembly. This highlights IRSp53 as a crucial host factor in HIV-1 membrane curvature and its requirement for full HIV-1 particle assembly.
Journal Title
Elife
Conference Title
Book Title
Edition
Volume
10
Issue
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
© 2021, Inamdar et al. This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Item Access Status
Note
Access the data
Related item(s)
Subject
Biochemistry and cell biology
physics of living systems
viruses
Persistent link to this record
Citation
Inamdar, K; Tsai, F-C; Dibsy, R; de Poret, A; Manzi, J; Merida, P; Muller, R; Lappalainen, P; Roingeard, P; Mak, J; Bassereau, P; Favard, C; Muriaux, DM, Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53, Elife, 2021, 10, pp. e67321