Characterizing Embryonic Gene Expression Patterns in the Mouse Using Nonredundant Sequence-Based Selection

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Sousa-Nunes, Rita
Rana, Amer Ahmed
Kettleborough, Ross
M. Brickman, Joshua
Clements, Melanie
Forrest, Alistair
Grimmond, Sean
Avner, Philip
Smith, James
L .Dunwoodie, Sally
S. P. Beddington, Rosa
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2003
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Abstract

This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.

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Genome Research

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13

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12

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© 2003 Cold Spring Harbor Laboratory Press. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.

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Biological Sciences

Medical and Health Sciences

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