Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology

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Gorgannezhad, Lena
Stratton, Helen
Nam-Trung, Nguyen
Griffith University Author(s)
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2019
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Abstract

Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples. Microfluidic devices allow for rapid advances in microbiology, enabling access to methods of amplifying nucleic acid molecules and overcoming difficulties faced by conventional. In this review, we summarize the recent progress in microfluidics-based polymerase chain reaction devices for the detection of nucleic acid biomarkers. The paper also discusses the recent development of isothermal nucleic acid amplification and droplet-based microfluidics devices. We discuss recent microfluidic techniques for sample preparation prior to the amplification process.

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Micromachines

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10

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6

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© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

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Nanotechnology

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Nanoscience & Nanotechnology

Instruments & Instrumentation

Science & Technology - Other Topics

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Gorgannezhad, L; Stratton, H; Nam-Trung, N, Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology, Micromachines, 2019, 10 (6)

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