A new strategy to enable the fabrication of a highly stable lithium nanowire network on titanium (Ti) surfaces is presented. The Ti surfaces were chemically modified by an alkali treatment, followed by in situ transformation of alkali-titanate into a Li-nanowire network (Ti-Li) via ionic exchange of Li+ ions during the hydrothermal reaction. The physicochemical characterization of the as-prepared Ti-Li substrates were analyzed using FE-SEM, XRD, LA-ICP-MS, and XPS techniques, in order to confirm the successful deposition of Li+ ions onto the Ti substrates. In-depth topographical and chemical characterization revealed that the stable continuous nanowire network is composed of fine Li-based nanoparticles (∼7 nm) and exhibits high surface wettability, high mechanical stability and a sustained release of Li+ ions over 21 days at 37 °C under vigorous shaking in Milli-Q water, simulated body fluid (SBF) and protein-containing fluids. Despite the coverage of the Ti-Li treated surface with a nanocrystals layer from the surrounding SBF media, Li release was not impaired. Human osteoblasts-derived cells cultured on the resultant Ti-Li surfaces indicated good viability, strong adhesion and attachment onto the nanowires. In conclusion, this novel Li-incorporated nano-scaled surface modification approach holds great promise towards the fabrication of bone/titanium dental implants with superior bone-forming ability.