This paper presents an integration framework for image segmentation. The proposed method is based on Fuzzy c-means clustering (FCM) and level set method. In this framework, firstly Chan and Vese's level set method (CV) and Bayes classifier based on mixture of density models are utilized to find a prior membership value for each pixel. Then, a supervised kernel based fuzzy c-means clustering (SKFCM) algorithm assisted by prior membership values is developed for final segmentation. The performance of our approach has been evaluated using high-throughput fluorescence microscopy colon cancer cell images, which are commonly used for the study of many normal and neoplastic procedures. The experimental results show the superiority of the proposed clustering algorithm in comparison with several existing techniques.